Dr. Santosh R. Kanade

Central University of Kerala, Kerala

Speaker

Expertise: Protein Biochemistry, Cell Signalling, Epigenetics.

Bio:

Dr. Santosh R. Kanade carried his MSc in Biochemistry at Karnatak University, Dharwad and PhD Biochemistry in University of Mysore (CFTRI), Mysore. Later started his career as postdoctoral research fellow in Case Western Reserve University Cleveland, OH, USA and University of Maryland Baltimore, MD, USA. Presently he is working as assistant Professor in Biochemistry at Central University of Kerala.

Presentation :

Title : A novel L-fucose-binding lectin induced cytotoxicity in cancer cells

Abstract :

Lectins are carbohydrate-binding proteins, which are ubiquitously expressed with nonimmune origin. The living organisms have complex heterogeneic carbohydrates with molecular pattern on their cell surface. The structural variability and functional versatility of cell surface glycans allow them to function as signaling, recognition and adhesion molecules. The human glycome has major interest in cancer research because of the aberrant glycosylation observed in all types of human cancers and a vast array of glycosyl epitopes is classified as tumor-associated carbohydrate antigens. The lectins, their involvement in a large number of cellular processes and their unique recognition ability initiated the development of various tools and technologies that utilize lectins in decoding and recognizing specific carbohydrates. In this study, we purified a fucose specific-lectin from marine organism using fucose-affinity column and characterized for its haemagglutination activity, carbohydrate specificity, dependency on cations and cytotoxicity against cancer cells. The lectin showed non-specificity against human erythrocytes. It was a Ca2+-dependent lectin that remained stable over wide pH and temperature ranges. The lectin exhibit dose dependent cytotoxicity against different human cancer cell lines and induced apoptosis as evidenced by DNA ladder assay and PARP cleavage. The increased level of p21 and corresponding downregulation of cyclin D in response to lectin treatment was observed which might work as probable factors to inhibit cell growth and induce apoptosis. We report a novel lectin from the haemolymph with high specificity for L-fucose and antiproliferative towards human cancer cells. However, further establishment of the modus operandi of this lectin is required to enable its biotechnological applications

Related Journals & Conferences :

International Biotechnology and Pharmaceutical Industry Forum