Dr.Somayeh Afsah Vakili

Dr.Somayeh Afsah Vakili |Clyto Access

Visveswarapura Institute of Pharmaceutical Sciences, Bangalore, India

Poster Presentation

Expertise: Cancer therapy

Biography:

Dr. Somayeh Afsah vakili recently completed PhD in pharmacology field from Visveswarapura Institute of Pharmaceutical Sciences, Bangalore, India which is affiliated by Rajiv Gandhi University of Health Science. She studied B.Pharm, M.Pharm and PhD at RGUH university, Bangalore, India. She also have Diploma degree in an Agriculture from Jondishapor university, Ahvaz, Iran. She is a member of editorial board of Advance Journal of Pharmacie and Life Science Research. She is an associate member of the Association of Pharmaceutical Teacher of India, 2010 onwards. She has a publication in Global Journal of medical research, American Journal of Pharmatech Research, Latin American Journal of Pharmacy, European Journal of Pharmaceutical and Medical Research, International Journal of Advanced Research, World Journal of Pharmaceutical Research and Advance Journal of Pharmacie and Life Science Research.

Presentation:

Title: Synergic antitumor activity of Ziziphus mauritiana and Punica granatum L on the inhibition of HeLa cell proliferation and tumor growth in EAC bearing mice

Abstract:

Since the increase in the use of synthetic chemicals in cancer therapy has led to many side effects and undesirable hazards, there is a worldwide trend to go back to natural resources, which are therapeutically effective, culturally acceptable and economically within the reach of the poor people. In the last few years, development of phenolic compounds or extracts from different plants has become a major area of health and medical research due to their marked effects in the prevention of various oxidative stress associated disease such as cancer. The previous researches have been revealed their cancer preventive properties through induction apoptosis. The present study was designed to investigate the synergic anticancer effect of Punica granatum L and Ziziphus mauritiana for invitro cytotoxicity, activation of caspase 8 and 9, express proapoptotic mediator p53, ant-iapoptotic protein Bcl-2 and DNA fragmentation. The data revealed that ethanol extract of combination of both plants were found to be the most effective in BSL assay, trypan blue exclusion assay and also stimulated caspase- 8 and-9 protease by 17.7-fold increase in Ac-IETD-pNA and 18.9-fold increase in Ac-LEHD-pNA respectively. The expression of proapoptotic and cell cycle arrest p53 in cytoplasm and nuclei and the amount of TdT in the nuclei of Ehrlich ascites carcinoma cells in mice treated with ethanol extract of combination of both plants were remarkably increased while the anti-apoptotic protein Bcl-2 expression was decreased. Consequently, the synergic anticancer activity of ethanol extract of Punica granatum L and Ziziphus mauritiana was proved.

Related Conferences :

International Biotechnology and Pharmaceutical Industry Forum