Sajad Majeed Zargar

Sajad Majeed Zargar |Clyto Access

Sher-e-Kashmir University of Agricultural Sciences and Technology, India

Speaker

Expertise: OMICS Insights into Common Bean (Phaseolus vulgaris L.) under Mineral Stress

Biography:

Sajad Majeed Zargar, Ph.D, is currently an Assistant Professor at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-Kashmir) in India. He was previously a Visiting Professor at the Nara Institute of Science and Technology, Japan. He has worked as an Assis-tant Professor at SKUAST-Jammu, Baba Ghulam Shah Badshah Univer¬sity (BGSB) in India

Presentation:

Title: OMICS Insights into Common Bean (Phaseolus vulgaris L.) under Mineral Stress

Abstract:

Common bean (Phaseolus vulgaris L.) is a valuable legume food crop nutritionally as well as medicinally. Being a good quality and cheap source of protein, fiber, carbohydrates, minerals and vitamins it has the potential to play a leading role in addressing the issues of malnutrition and hunger-related problems for the poor of the world. As a consequence, it is rightly considered as the “poor man’s meat”. Among various minerals that common bean possesses, iron (Fe) and zinc (Zn) are present in relatively high amount and are important for both plants and animals for a range of critical enzymatic and metabolic reactions. Fe is required for basic redox reactions in both photosynthesis and respiration, and for many key enzymatic reactions involved in important biological processes such as DNA replication, lipid metabolism and nitrogen fixation in plants. Zn is also necessary for various metabolic and physiological processes in plants acting as a cofactor for more than 300 enzymes. Common bean is considered as a legume model to unravel the responses and adaptation to Fe, Zn and P deficiencies1.

In our study, we have used GWAS (Genome Wide Association) approach to identify QTLs contributing for accumulation of Fe and Zn in common bean seeds. Further, we tried to examine the impact of Fe deficiency and excess Zn on the leaf proteome of 15-days-old common bean seedlings (variety VLR-125) using gel-based proteomics (2-DE) in conjunction with MALDI-TOF/MS analysis. Physiological and morphological parameters revealed that Fe deficiency and excess Zn had similar impact on chlorophyll content of shoots, shoot and root length and weight compared to the control condition. For proteomics analysis, selected proteins that were differentially expressed under Fe deficiency and excess Zn compared to control (basal MGRL medium) were studied in detail. A total of 46 proteins were found up-regulated and 26 proteins down-regulated in Fe deficiency compared to control. Similarly, nine proteins were observed up-regulated and six proteins were down-regulated in excess Zn condition compared to the control. Further, seven proteins were observed up-regulated and five proteins down-regulated under both Fe deficiency and excess Zn conditions, indicating the possibility of cross talk under such conditions. Based on cellular compartmentalization, most of the identified proteins were found to be localized to the chloroplast and nucleus, whereas only few identified proteins where found localized in mitochondria, endoplasmic reticulum and plasma membrane. These proteins were found to be involved in various biological functions like carbohydrate metabolism, photosynthesis, defense and stress response, transport, cellular processes (replication, transcription and translation) and cellular respiration. This work provides a comprehensive model to understand the adaptive mechanism used by common bean shoots under Fe deficiency/excess Zn conditions at the protein level. However, further, in-depth proteome analysis is required to understand the cross talk under Fe deficiency and excess Zn in common bean.

References

1 Castro-Guerrero NA, Isidra-Arellano MC, Mendoza-Cozatl DG and Valdés-López O. 2016 Common Bean: A Legume Model on the Rise for Unraveling Responses and Adaptations to Iron, Zinc, and Phosphate Deficiencies. Front. Plant Sci. 7:600

Acknowledgement:

This work was partially supported by SERB, DST, New Delhi (Ref. No.: SR/FT/LS-27/2011) fund to SMZ.

Related Conferences :

Agriculture and Crop Science Conference